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gene exp ebf3 mm00438642 m1  (Thermo Fisher)


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    Thermo Fisher gene exp ebf3 mm00438642 m1
    <t>Ebf3</t> regulatory landscape and associated hs737/Rr169617 enhancer deletion mouse lines. Genome browser view of the topologically associating domain region containing Rr169617 and its target gene Ebf3 (GRCm38/mm10). The first track shows the two independent founder mouse lines generated in this study: line 299 (C57BL/6J-Rr169617 em1Tnt /J) and line 304 (C57BL/6J-Rr169617 em2Tnt /J). The second track shows the location of the regulatory region, Rr169617. The third track shows the location of human VISTA enhancers lifted over to the mouse genome. Included is hs737 that resides within the Rr169617 region. The fourth track shows enhancer-promoter interactions of Rr169617 and Ebf3 from . The fifth track shows the genes within the region. The sixth track shows human topologically associating domains lifted over to this region and show high conservation. Finally, the chromatin state data available from ENCODE3 is shown across the different timepoints in mouse development. The colors in this track refer to their chromHMM status as described in .
    Gene Exp Ebf3 Mm00438642 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp ebf3 mm00438642 m1/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    gene exp ebf3 mm00438642 m1 - by Bioz Stars, 2026-04
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    1) Product Images from "Generation and characterization of a knockout mouse of an enhancer of EBF3"

    Article Title: Generation and characterization of a knockout mouse of an enhancer of EBF3

    Journal: Biology Open

    doi: 10.1242/bio.062070

    Ebf3 regulatory landscape and associated hs737/Rr169617 enhancer deletion mouse lines. Genome browser view of the topologically associating domain region containing Rr169617 and its target gene Ebf3 (GRCm38/mm10). The first track shows the two independent founder mouse lines generated in this study: line 299 (C57BL/6J-Rr169617 em1Tnt /J) and line 304 (C57BL/6J-Rr169617 em2Tnt /J). The second track shows the location of the regulatory region, Rr169617. The third track shows the location of human VISTA enhancers lifted over to the mouse genome. Included is hs737 that resides within the Rr169617 region. The fourth track shows enhancer-promoter interactions of Rr169617 and Ebf3 from . The fifth track shows the genes within the region. The sixth track shows human topologically associating domains lifted over to this region and show high conservation. Finally, the chromatin state data available from ENCODE3 is shown across the different timepoints in mouse development. The colors in this track refer to their chromHMM status as described in .
    Figure Legend Snippet: Ebf3 regulatory landscape and associated hs737/Rr169617 enhancer deletion mouse lines. Genome browser view of the topologically associating domain region containing Rr169617 and its target gene Ebf3 (GRCm38/mm10). The first track shows the two independent founder mouse lines generated in this study: line 299 (C57BL/6J-Rr169617 em1Tnt /J) and line 304 (C57BL/6J-Rr169617 em2Tnt /J). The second track shows the location of the regulatory region, Rr169617. The third track shows the location of human VISTA enhancers lifted over to the mouse genome. Included is hs737 that resides within the Rr169617 region. The fourth track shows enhancer-promoter interactions of Rr169617 and Ebf3 from . The fifth track shows the genes within the region. The sixth track shows human topologically associating domains lifted over to this region and show high conservation. Finally, the chromatin state data available from ENCODE3 is shown across the different timepoints in mouse development. The colors in this track refer to their chromHMM status as described in .

    Techniques Used: Generated

    qRT-PCR analysis for Ebf3 expression in E12.5 forebrain. (A) Results of five independent qRT-PCR for Ebf3 expression. Samples for each genotype were as follows: Rr169617 +/+ ( n =12), Rr169617 +/− ( n =14), and Rr169617 −/− ( n =10). (B) Relative fold expression aggregating data across all five independent qPCR experiments. For both A and B, relative fold expression is in comparison to the Rr169617 +/+ results.
    Figure Legend Snippet: qRT-PCR analysis for Ebf3 expression in E12.5 forebrain. (A) Results of five independent qRT-PCR for Ebf3 expression. Samples for each genotype were as follows: Rr169617 +/+ ( n =12), Rr169617 +/− ( n =14), and Rr169617 −/− ( n =10). (B) Relative fold expression aggregating data across all five independent qPCR experiments. For both A and B, relative fold expression is in comparison to the Rr169617 +/+ results.

    Techniques Used: Quantitative RT-PCR, Expressing, Comparison



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    Image Search Results


    Ebf3 regulatory landscape and associated hs737/Rr169617 enhancer deletion mouse lines. Genome browser view of the topologically associating domain region containing Rr169617 and its target gene Ebf3 (GRCm38/mm10). The first track shows the two independent founder mouse lines generated in this study: line 299 (C57BL/6J-Rr169617 em1Tnt /J) and line 304 (C57BL/6J-Rr169617 em2Tnt /J). The second track shows the location of the regulatory region, Rr169617. The third track shows the location of human VISTA enhancers lifted over to the mouse genome. Included is hs737 that resides within the Rr169617 region. The fourth track shows enhancer-promoter interactions of Rr169617 and Ebf3 from . The fifth track shows the genes within the region. The sixth track shows human topologically associating domains lifted over to this region and show high conservation. Finally, the chromatin state data available from ENCODE3 is shown across the different timepoints in mouse development. The colors in this track refer to their chromHMM status as described in .

    Journal: Biology Open

    Article Title: Generation and characterization of a knockout mouse of an enhancer of EBF3

    doi: 10.1242/bio.062070

    Figure Lengend Snippet: Ebf3 regulatory landscape and associated hs737/Rr169617 enhancer deletion mouse lines. Genome browser view of the topologically associating domain region containing Rr169617 and its target gene Ebf3 (GRCm38/mm10). The first track shows the two independent founder mouse lines generated in this study: line 299 (C57BL/6J-Rr169617 em1Tnt /J) and line 304 (C57BL/6J-Rr169617 em2Tnt /J). The second track shows the location of the regulatory region, Rr169617. The third track shows the location of human VISTA enhancers lifted over to the mouse genome. Included is hs737 that resides within the Rr169617 region. The fourth track shows enhancer-promoter interactions of Rr169617 and Ebf3 from . The fifth track shows the genes within the region. The sixth track shows human topologically associating domains lifted over to this region and show high conservation. Finally, the chromatin state data available from ENCODE3 is shown across the different timepoints in mouse development. The colors in this track refer to their chromHMM status as described in .

    Article Snippet: Taqman mouse Ebf3 (Mm00438642_m1) and GAPDH (Mm99999915_g1) gene expression assays were performed on a QuantStudio 6 Flex quantitative thermocycler using four reactions for each sample.

    Techniques: Generated

    qRT-PCR analysis for Ebf3 expression in E12.5 forebrain. (A) Results of five independent qRT-PCR for Ebf3 expression. Samples for each genotype were as follows: Rr169617 +/+ ( n =12), Rr169617 +/− ( n =14), and Rr169617 −/− ( n =10). (B) Relative fold expression aggregating data across all five independent qPCR experiments. For both A and B, relative fold expression is in comparison to the Rr169617 +/+ results.

    Journal: Biology Open

    Article Title: Generation and characterization of a knockout mouse of an enhancer of EBF3

    doi: 10.1242/bio.062070

    Figure Lengend Snippet: qRT-PCR analysis for Ebf3 expression in E12.5 forebrain. (A) Results of five independent qRT-PCR for Ebf3 expression. Samples for each genotype were as follows: Rr169617 +/+ ( n =12), Rr169617 +/− ( n =14), and Rr169617 −/− ( n =10). (B) Relative fold expression aggregating data across all five independent qPCR experiments. For both A and B, relative fold expression is in comparison to the Rr169617 +/+ results.

    Article Snippet: Taqman mouse Ebf3 (Mm00438642_m1) and GAPDH (Mm99999915_g1) gene expression assays were performed on a QuantStudio 6 Flex quantitative thermocycler using four reactions for each sample.

    Techniques: Quantitative RT-PCR, Expressing, Comparison